BMC Plant Biology

Table 6 Description of the molecular markers applied in the study for the identification of the allelic state ofRht,VRNandPpdgenes

From:Effects ofRht17in combination withVrn-B1andPpd-D1alleles on agronomic traits in wheat in black earth and non-black earth regions

Gene	Primer sequence	Allele	Expected size of the product, bp	Reference
Rht-B1	Rht-B1p-WF: 5′ ACATGGCGGACGTGGTGC 3′ Rht-B1-R1: 5′ GCCGAGAGAGGACGAT 3’	Rht-B1a	425	[7,66]
	Rht-B1p-F: 5’ ACATGGCGGACGTGGTGT 3′ Rht-B1-R1: 5′ GCCGAGAGAGGACGAT 3’	Rht-B1p^a (Rht17)	425	[7,66]
	BF: 5’ GGTAGGGAGGCGAGAGGCGAG 3′ WR1: CATCCCCATGGCCATCTCGAGCTG	Rht-B1a	237	[67]
	BF: 5′ GGTAGGGAGGCGAGAGGCGAG 3′ MR1: 5′ CATCCCCATGGCCATCTCGAGCTA 3′	Rht-B1b^a (Rht1)	237	[67]
	BF: 5′ GGTAGGGAGGCGAGAGGCGAG 3′ W3: 5′ GGCCATCTCCAGCTGCTCCAGCTT 3’	Rht-B1a	228	[66]
	BF: 5’ GGTAGGGAGGCGAGAGGCGAG 3′ M3: 5′ GGCCATCTCCAGCTGCTCCAGCTA 3’	Rht-B1e^a (Rht11)	228	[66]
Rht-D1	DF2: 5’- GGCAAGCAAAAGCTTCGCG −3′ WR2: 5’- GGCCATCTCGAGCTGCAC −3’	Rht-D1a	264	[67]
Rht-D1	DF: 5’- CGCGCAATTATTGGCCAGAGATAG − 3′ MR2: 5’- CCCCATGGCCATCTCGAGCTGCTA − 3’	Rht-D1b^a (Rht2)	254	[67]
Ppd-D1	Ppd-D1_F: ACGCCTCCCACTACACTG Ppd-D1_R2: CACTGGTGGTAGCTGAGATT Ppd-D1_R1: GTTGGTTCAAACAGAGAGC	Ppd-D1a	288	[68]
Ppd-D1		Ppd-D1b^a	414	[68]
VRN-A1	VRN1AF: GAAAGGAAAAATTCTGCTCG VRN1-INT1R: GCAGGAAATCGAAATCGAAG	Vrn-A1a	965 + 876	[69,70]
		Vrn-A1b	714
	VRN1AF: GAAAGGAAAAATTCTGCTCG VRN1-INT1R: GCAGGAAATCGAAATCGAAG	vrn-A1^a	734
VRN-B1	Intr1/B/F: CAAGTGGAACGGTTAGGACA Intr1/B/R3: CTCATGCCAAAAATTGAAGATGA	Vrn-B1a	709	[70]
	Intr1: ATCATCTTCTCCACCAAGGG Intr1/B/R3: CTCATGCCAAAAATTGAAGATGA	Vrn-B1a Vrn-B1c	1124 737	[71]
	Intr1/B/F: CAAGTGGAACGGTTAGGACA Intr1/B/R4: CAAATGAAAAGGAATGAGAGCA	vrn-B1^a	1149
VRN-D1	Intr1/D/F: GTTGTCTGCCTCATCAAATCC Intr1/D/R3: GGTCACTGGTGGTCTGTGC	Vrn-D1	1671	[70]
VRN-D1	Intr1/D/F: GTTGTCTGCCTCATCAAATCC Intr1/D/R4: AAATGAAAAGGAACGAGAGCG	vrn-D1^a	997	[70]
Rht-8	WMS261-F: 5’- CTCCCTGTACGCCTAAGGC −3′ WMS261-R: 5’- CTCGCGCTACTAGCCATTG − 3′	Rht-8c	192	[72]

^arecessive (or partially recessive) allele

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