Fig. 5

Comparison of allele discrimination in KASP and Amplifluor-like systems using the same Master-mix. SNP genotyping was developed targeting SNPs in a barley collection, in theFT5gene using KASP (a) and Amplifluor-like (b) systems; and in thePHY-Cgene using the same KASP (c) and Amplifluor-like (d) systems. Blue and red dots indicate genotypes with homozygous alleles, green dots for heterozygous, pink dots and crosses for samples with no signals, and black dots and squares shows NTC, No template controls. X- and Y-axes show Relative amplification units, ΔRn, for FAM and HEX/VIC fluorescence signals, respectively, as determined by the qPCR instrument