From:Taxonomic assessment ofAlliumspecies from Kazakhstan based on ITS andmatKmarkers
| Primers | Nucleotide sequence | Annealing temperature | Amplicon sizes |
|---|---|---|---|
| ITS1nF ITS4nR |
5’-AGAAGTCGTAACAAGGTTTCCGTAGG- 3′ 5’-TCCTCCGCTTATTGATATGC- 3’ |
58 °C | 638 bp |
| matK-F matK-R |
5’-CCTATCCATCTGGAAATCTTAG- 3′ 5’-GTTCTAGCACAAGAAAGTCG- 3’ |
50 °C | 788 bp |