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Table 2 Gene-specific primers used for amplification of barley DNA. For primer design, the contigs sequences mentioned in Table1were used. Primer pairs used for promoter region sequencing are shaded by a grey colour

From:Identification and characterization of regulatory network components for anthocyanin synthesis in barley aleurone

  1. *For molecular mapping, amplified fragments of the mapping population individuals were digested with restriction endonucleasesBse1我,HgaI andEcoRI respectively (CAPS –analysis)