Fig. 6

qRT-PCR analysis of GhmiR157 andGhSPLexpression patterns.aNorthern blot analysis of mature GhmiR157 expression during floral organ bud developmental stages.bNorthern blot analysis of mature GhmiR157 expression in different tissues. R, H, L, P, An, St, 0O, 3O, 5 F, 5O represent the root, hypocotyl, leaf, petal, anther, stigma, 0 DPA ovule, 3 DPA ovule, 5 DPA fibre, and 5 DPA ovule, respectively.c-mQuantitative analysis ofGhSPLexpression in different floral bud developmental stages. R.E.L., the relative expression levels calculated usingHISTONE3(AF024716) as a control. The error bars indicate the standard deviation of three biological replicates. Different letters in the plots indicate statistically significant differences atP < 0.05 based on analysis of variance (ANOVA) (Tukey’s multiple comparison test).nImages of longitudinal sections of different length (mm) of floral buds and opened flowers. The numbers 0–2, 2–4, 4–6, 6–8, 8–10, 10–16, 16–25 represent different lengths (mm) of floral buds