一氧化氮，植物细胞再激活中的信号分子

已知一氧化氮可作为动物生物中潜水信号转导途径中的生物信使。初步调查表明，植物通过与哺乳动物中发现的那些相似的途径使用一氧化氮作为信号分子。特别是，在植物防御反应期间NO的突然角色是很好的。然而，安装证据支持假设，即NO是植物生长和发展的更普遍效应。

在我们的实验室中,苜蓿细胞培养to investigate the possible involvement of NO in the regulation of cell division and differentiation in plant cells. The homogenous population of leaf protoplasts were cultured in the presence of a NO donor, sodium nitroprusside (SNP) and/or an inhibitor, NG-monomethyl-L-arginine (L-NMMA). BrdU incorporation frequency into the nuclei of the protoplast-derived cells indicated that the entry into the S-phase of the cell cycle is enhanced by SNP and inhibited by L-NMMA treatments, respectively (see Figure1）。还通过连续分割细胞悬浮培养物进行实验。所获得的数据表明，这些类型的细胞对显示的类似治疗不敏感，以影响原生质体衍生的细胞分裂（见图2）。

BrdU incorporation frequency into the nuclei of leaf protplast-derived alfalfa cells during the third day of culture. The cells were treated by the indicated drugs affecting endogenous NO formation. For cell culture details see [2].

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BrdU incorporation frequency into the nuclei of cell suspension-cultured alfalfa cells during the third day after subculture. The cells were treated by the indicated drugs affecting endogenous NO formation. For cell culture details see [2].

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除了细胞周期进展外，上述药物对叶片原生质体的胚胎源性富集细胞的形成的影响（有关详细信息，请参见[1那2]已经遵循了。在该方法上观察到SNP的促进作用和L-NMMA的抑制作用，特别是在低外源性助产毒素（0.02μm2,4-二氯苯酸乙酸）补充。